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This volume presents readers with a collection of detailed protocols for the detection, quantification, and functional analysis of H¿S and related sulfur species in biological systems. The chapters in this book cover a broad range of approaches such as accessible colorimetric and fluorometric assays, fluorescence microscopy, flow cytometry, extracellular flux analysis, chemoselective derivatization, and liquid chromatography-tandem mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Hydrogen Sulfide Signaling: Methods and Protocols is a valuable resource for investigators studying sulfur metabolism, sulfide bioavailability, enzymatic H¿S generation, donor release kinetics, mitochondrial responses, and protein persulfidation.
This volume presents readers with a collection of detailed protocols for the detection, quantification, and functional analysis of H¿S and related sulfur species in biological systems. The chapters in this book cover a broad range of approaches such as accessible colorimetric and fluorometric assays, fluorescence microscopy, flow cytometry, extracellular flux analysis, chemoselective derivatization, and liquid chromatography-tandem mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Hydrogen Sulfide Signaling: Methods and Protocols is a valuable resource for investigators studying sulfur metabolism, sulfide bioavailability, enzymatic H¿S generation, donor release kinetics, mitochondrial responses, and protein persulfidation.
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